How to deposit microbial resources in NCVTC Repository
- Contact the concerned scientific staff in NCVTC (see contact details at the back) before depositng microbial cultures in the repository.
- Download the preliminary culture data sheet from the Centre’ website (http:// www.nrce.nic.in ; http://vtcc.gov.in )
- Send the microbial cultures alongwith the duly filled preliminary culture data sheet to the curator.
- Repository receipt numbers will be communicated to the depositors for those cultures which pass the preliminary quality testing.
- Accession numbers shall be communicated after authentication of the cultures, subject to the submission of the duly filled deposit form. The accessioning process may take between 10-12 weeks.
• Bacteria: The bacterial cultures can be deposited in freeze dried form or as slant and may be transported on ice.
• Virus: The virus cultures should be deposited preferably in freeze dried form (10 nos) or as frozen cultures (10 X 2ml cryovials) and transported preferably in dry ice / liquid nitrogen.
• Plasmid/genomic DNA : Plasmid/genomic DNA should be deposited in both liquid form in TE buffer & precipitate form in 80% ethanol. Amount of genetic material: a) plasmid: 200µl ( 50ng/µl) in TE & ~1µg in 80% ethanol; b) genomic: 200µ (100-200ng/ul) in TE & ~5ug in 80% ethanol.
• Recombinant clones: Should be submitted as described under deposit form for recombinant clones
• Bacteriophage: The bacteriophages should be deposited in SM buffer and corresponding host in freeze dried form/stab in slant