Rumen Microbes

  1. To isolate cellulolytic rumen bacteria from Indian cattle, sheep, goats and buffaloes.
  2. To isolate rumen fungi from domesticated and wild animals.
  3. To determine the best fibre digesting organism.
  4. To clone the cellulase gene from the best fibre digesting organism into a suitable target bacteria.

Dairy Microbes

  1. To isolate indigenous strains of lactic acid bacteria including the ones with probiotic attributes from ethnic fermented milks and other foods prepared in various parts of India
  2. To identify and characterize the isolated strains using conventional and molecular techniques based on 16S rRNA sequencing, PCR (Real time), DNA probes and Molecular Beacons / DNA Micro-assay.
  3. To analyse the plasmid pool encoding commercially important traits of selected cultures and their characterization at molecular level.
  4. To construct new recombinants with different phenotypes by gene transfer systems
  5. To clone and sequence commercially important genes of lactic acid bacteria particularly related to probiotic functions
  6. To identify the commercial potential of these diversified strains for designing novel starter cultures with functional / probiotic properties
  7. To conserve these strains and their germplasm ex situ and create a well catalogued.
  8. To establish a national culture bank of indigenous lactic strains at NDRI, Karnal

Veterinary Microbes

  1.  Exploration and collection of microbes of animal origin / significance / relevance.
  2. Identification and characterization the isolated / collected microbial pathogens from different disease conditions and carrier animals by conventional and molecular techniques.
  3. Collection and central storage of animal microbes from existing culture collections, institutions and universities.
  4. To clone and sequence important genes to generate epidemiological information and to understand disease pathogenesis
  5. To create a genome/gene bank of different microbes by storing genomic DNA/RNA, recombinant plasmid clones, cell lines, bacteria, virus, bacteriophages etc for conservation of microbial biodiversity
  6. To procure and deposit reference microbial strains/isolates, vaccine strains, plasmids, genomic DNA/RNA, clones etc
  7. To reposit and supply biological materials for validating assays.
  8. To identify suitable isolate/strains for development of vaccines.
  9. Documentation and digitization of microbial database of cultures of veterinary microbes.